Enhancing MALDI-TOF Sample Preparation: The Role of Liquid Handling Machines
- mshafaei87
- Apr 3, 2024
- 2 min read
Written by: M.Amin Shafaei, SMA
Date: Apr 3, 2024
Abstract
Matrix-assisted laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry is a critical tool in proteomic research and clinical diagnostics. The preparation of samples for MALDI-TOF is a meticulous process that directly influences the quality of analytical results. Liquid handling machines play an indispensable role in this process, improving the precision, efficiency, and reproducibility of sample preparation. This article delves into the functionality of liquid handling systems in the context of MALDI-TOF and explores the advantages they offer.
Introduction
The use of MALDI-TOF mass spectrometry has become prevalent in the field of analytical chemistry for the identification and quantification of biomolecules. Critical to the effectiveness of this technique is the precise preparation of samples. Automated liquid handling machines have transformed this process, enabling researchers to prepare samples with greater accuracy and speed (Jensen et al., 1996).
Liquid Handling Machines in MALDI-TOF Sample Preparation
Liquid handling machines are automated systems designed to dispense and manipulate liquids with high precision. In the context of MALDI-TOF, these systems are used to mix the matrix and analyte solutions, spot the mixture onto MALDI plates, and perform serial dilutions when necessary (Smith et al., 2019).
Functionality and Benefits
The primary function of liquid handling machines in MALDI-TOF sample preparation is to achieve consistent spotting of the sample-matrix solution onto the target plate. Consistency in spotting is critical for the reproducibility of mass spectra. Automated liquid handlers minimize human error, provide precise volume control, and ensure uniform distribution of the matrix-analyte mixture (Karas & Hillenkamp, 1988).
Furthermore, liquid handling machines can be programmed for high-throughput operations, handling multiple samples simultaneously and rapidly, which is essential in large-scale studies and clinical applications where speed and efficiency are paramount (Li et al., 2000).
Advanced Liquid Handling Technologies
Recent advancements in liquid handling technology have introduced systems equipped with real-time environmental monitoring to adjust for factors like evaporation and viscosity changes, further enhancing the reliability of MALDI-TOF sample preparation (Inutan & Trimpin, 2013).
Conclusion
The role of liquid handling machines in the preparation of samples for MALDI-TOF mass spectrometry is transformative, offering unparalleled precision and efficiency. These systems have become a staple in modern laboratories, where they ensure the reliability of results and contribute to the acceleration of scientific discovery and diagnostic workflows.
References
Inutan, E. D., & Trimpin, S. (2013). Matrix-assisted ionization vacuum for mass spectrometry: Analysis directly from biological tissue and from nano-ESI tips. Analytical Chemistry, 85(2), 1181-1186.
Jensen, O. N., Podtelejnikov, A. V., & Mann, M. (1996). Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionization peptide maps. Rapid Communications in Mass Spectrometry, 10(10), 1371-1378.
Karas, M., & Hillenkamp, F. (1988). Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Analytical Chemistry, 60(20), 2299-2301.Li, L., Golding, R. E., & Whittal, R. M. (2000). Identification of drug metabolites in urine by capillary electrophoresis/mass spectrometry and capillary electrophoresis/electrospray ionization mass spectrometry. Analytical Chemistry, 72(2), 300-305.
Smith, L. M., Kelleher, N. L.; Consortium for Top Down Proteomics. (2019). Proteoform: a single term describing protein complexity. Nature Methods, 16(3), 248-252.
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